7/31/2023 0 Comments Noti hf neb![]() ![]() Although the propensity for star activity varies, the vast majority of enzymes from New England Biolabs will not exhibit star activity when used under recommended conditions in their supplied NEBuffers. It has been suggested that star activity is a general property of restriction endonucleases (1) and that any restriction endonuclease will cleave noncanonical sites under certain extreme conditions, some of which are listed below. This altered specificity has been termed “star activity”. Under non-standard reaction conditions, some restriction enzymes are capable of cleaving sequences which are similar, but not identical, to their defined recognition sequence. Whether you are setting up digests for 5-15 minutes or overnight, or using varying amounts of enzymes, HF enzymes ensure the performance you need. High Fidelity Restriction Enzymes have been engineered by exchanging functional amino acid residues and then screening for optimal mutants that perform under a wide range of conditions. Optimized performance for a wide range of conditions These engineered enzymes have the same specificity as the native enzyme, with the added benefit of reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart ® Buffer.Įngineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. The respected leader in the field of restriction enzyme biology, NEB has developed a line of High-Fidelity (HF ®) Restriction Enzymes. For over 35 years, New England Biolabs has been developing innovative solutions for molecular biology applications. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |